Vimentin antibody

Catalog No. ABIN6940881

This anti-Vimentin antibody is a Mouse Monoclonal antibody detecting Vimentin in IHC and StM. Suitable for Human, Mouse, Rat, Pig, Cow, Cat, Rabbit and Sheep.

Quick Overview for Vimentin antibody (ABIN6940881)

Target: Vimentin (VIM)

Reactivity: Human, Mouse, Rat, Pig, Cow, Cat, Rabbit, Sheep

Host: Mouse

Clonality: Monoclonal

Conjugate: This Vimentin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Clone: LN-6

Product Details anti-Vimentin Antibody

Specificity: This MAb reacts with a 58 kDa protein identified as vimentin. It reacts with a non-hematopoietic epitope of vimentin and shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Vimentin is ubiquitously expressed in mesenchymal cells such as fibroblasts, smooth muscle cells, and endothelium. Antibody against vimentin is useful as part of an antibody panel for differential diagnosis of tumors of unknown origin.Ab-2 does not react with leukocyte common antigen-positive tissues such as lymphomas and leukemias.

Immunogen: Human thymic nuclear extract

Isotype: IgM kappa

Application Details

Application Notes:

Positive Control: Jurkat cells, Sarcomas, Melanomas.

Known Application: Immunohistochemistry (Formalin-fixed) (0.1-0.2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Vimentin

Target: Vimentin (VIM)

Alternative Name: VIM

Molecular Weight: 57-60kDa

Gene ID: 7431

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis